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KMID : 0381920010310020199
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Korean Journal of Microscopy
2001 Volume.31 No. 2 p.199 ~ p.206
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Immunocytochemical Investigation on the Intracisternal Accumulations of Storage Protein in Pea Cotyledon Cells
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Jeong Byung-Kap
Park Hong-Duck
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Abstract
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In 1980s, the fragmentation or subdivision of protein deposits at the periphery of protein storage vacuole was suggested as the only route of PB development in pea cotyledon cells. Since then, other independant processes such as terminal dilation , transformation and de novo development have been discussed as alternative routes for PB development, and today, these multiple mechanisms of PB development are accepted as a result of active investigations. For analysis of the protein accumulations in the ER cisternae during seed development, immunocytochemical gold labellings were applyed on the single cells separated by enzymatic digestion from cotyledon tissue. Anti-legumin labellings at the early stage, and anti-vicilin labellings at the intermediate stage were observed on the protein-filled ER. The $\alpha-Tip$, which is the ER retention protein, was labelled somewhat at late stage, and PPase, a sort of tonoplast membrane protein, was labelled at early stage.
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KEYWORD
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$\alpha-Tip$, Immunocytochemistry, Legumin, Pea, PPase, Protein-filled ER, Vicilin
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